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| Registros recuperados : 175 | |
| Registros recuperados : 175 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
12/09/2022 |
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Data da última atualização: |
12/04/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T. |
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Afiliação: |
PAULA CRISTINA DA SILVA ANGELO, CNPCa. |
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Título: |
Coffee microspore cultivation to attain doubled-haploid stable plantlets. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França. |
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DOI: |
10.17660/ActaHortic.2023.1359.14 |
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Idioma: |
Inglês |
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Notas: |
E-poster. |
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Conteúdo: |
The aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli produced by the interspecific hybrid anthers. Regarding the hybrid anthers, 25% were infected by a same endogenous bacteria but 8% produced embryogenic tissue, with globular embryos multiplying and maturing simultaneously upon transfer to N6 medium without growth regulators. After 1.2 years, the photomorphogenesis has been induced under light, and auxin/cytokinin ratio = 2 plus GA3. MenosThe aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli prod... Mostrar Tudo |
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Thesagro: |
Café; Embriogénese; Pólen; Rubiaceae. |
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Thesaurus NAL: |
Embryogenesis; Pollen. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/249059/1/Coffee-microspore-cultivation.pdf
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Marc: |
LEADER 02577nam a2200241 a 4500
001 2146372
005 2023-04-12
008 2022 bl uuuu u00u1 u #d
024 7 $a10.17660/ActaHortic.2023.1359.14$2DOI
100 1 $aANGELO, P. C. da S.
245 $aCoffee microspore cultivation to attain doubled-haploid stable plantlets.$h[electronic resource]
260 $aINTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.$c2022
500 $aE-poster.
520 $aThe aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli produced by the interspecific hybrid anthers. Regarding the hybrid anthers, 25% were infected by a same endogenous bacteria but 8% produced embryogenic tissue, with globular embryos multiplying and maturing simultaneously upon transfer to N6 medium without growth regulators. After 1.2 years, the photomorphogenesis has been induced under light, and auxin/cytokinin ratio = 2 plus GA3.
650 $aEmbryogenesis
650 $aPollen
650 $aCafé
650 $aEmbriogénese
650 $aPólen
650 $aRubiaceae
700 1 $aPEREIRA, L. F. P.
700 1 $aSERA, G. H.
700 1 $aSETA, T.
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Registro original: |
Embrapa Café (CNPCa) |
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